| 25/09/2010 | 16:00 | Gala Fairydean - Sunnybank FC | - | |
| 25/09/2010 | 16:00 | Selkirk - Bo'ness United | - | |
| 25/09/2010 | 16:00 | Huntly - Girvan FC | - | |
| 25/09/2010 | 16:00 | Vale of Leithen - Keith | - | |
| 25/09/2010 | 16:00 | Lossiemouth - Whitehill Welfare | - | |
| 25/09/2010 | 16:00 | Deveronvale - Inverurie Loco Works | - | |
| 25/09/2010 | 16:00 | Edinburgh City - Brora Rangers | - | |
| 25/09/2010 | 16:00 | Newton Stewart FC - Preston Athletic | - | |
| 25/09/2010 | 16:00 | Hawick Royal Albert - Dalbeattie Star | - | |
| 25/09/2010 | 16:00 | Fraserburgh - St Cuthbert Wanderers | - | |
| 25/09/2010 | 16:00 | Coldstream - Forres Mechanics | - | |
| 25/09/2010 | 16:00 | Glasgow University - Burntisland Shipyard | - | |
| 25/09/2010 | 16:00 | Beith - Linlithgow Rose | - | |
| 25/09/2010 | 16:00 | Edinburgh City - Clachnacuddin | - | |
| 25/09/2010 | 16:00 | Civil Service Strollers - Wigtown & Bladnoch FC | - | |
| 25/09/2010 | 16:00 | Rothes - Nairn County | - | |
| 25/09/2010 | 16:00 | Golspie Sutherland - Fort William | - |
Beker van Schotland
